I provide comprehensive PCR primer design services for researchers, laboratories, and biotech companies, offering precise and optimized solutions for gene amplification, diagnostics, and molecular biology research. My service ensures that primers are specifically tailored to your target DNA or RNA sequences, minimizing non-specific amplification and maximizing reaction efficiency.
The process begins with an in-depth analysis of the target sequence, including gene structure, GC content, melting temperature (Tm), secondary structures, and potential primer-dimer formation. Using industry-standard bioinformatics tools such as Primer3, OligoAnalyzer, and NCBI BLAST, I design primers that are highly specific to the region of interest while avoiding off-target binding. Whether the goal is conventional PCR, quantitative real-time PCR (qPCR), or TaqMan probe-based assays, I can design primers suitable for your experimental needs.
I also provide primers compatible with various experimental conditions, including different annealing temperatures, product sizes, and DNA templates (genomic DNA, cDNA, or plasmid DNA). For qPCR assays, I can design forward and reverse primers along with probes that ensure high specificity, reproducibility, and sensitivity for accurate gene expression or pathogen detection.
Additionally, I offer guidance on primer validation, reaction optimization, and troubleshooting, helping clients save time and resources in the lab. My service is ideal for research in genetics, molecular diagnostics, microbiology, and biotechnology, supporting both academic and commercial projects.
With a strong background in molecular biology, bioinformatics, and genetic research, I ensure that every primer is designed to meet the highest standards of specificity, efficiency, and experimental success. I can deliver primer sets along with detailed documentation, including sequence information, expected product size, Tm, GC content, and in silico validation results, providing a complete solution for your PCR-based experiments.